5th Annual WSU Celebration of Research and Creative Scholarship
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Poster #22 - Derivatization and Measurement of Triclosan to Determine Extent of Removal by Common Wastewater Treatment Methods Megan Carlson Faculty Co-author: Dr. Jeanne Franz Triclosan or 5-chloro-2-(2,4-dichlorophenoxy)phenol, is a broad spectrum antimicrobial used in many commercial household products. An analytical method using liquid chromatography paired with fluorescence detection to determine concentrations of triclosan in wastewater was attempted. A scheme to derivatize triclosan was developed and a fluorimeter was used to determine the excitation and emission wavelengths. The chromatography conditions were developed and optimized by liquid chromatography tandem mass spectrometry, as the original instrument was not in working order. An isocratic condition of 70 % acetonitrile and 30 % water in 0.1 % formic acid at 0.2 ml/min was used. It was determined that triclosan was linear over three orders of magnitude and the detection limit was in the 5 – 10 ppb range. This is a considerable improvement over past methods of using UV detection, where the detection limit was approximately 20 ppm and is closer to levels found in the environment. Future research holds collecting wastewater samples and concentrating them to levels able to be detected. The samples will also be spiked and treated by the derivatization step to make sure the proper reaction takes place. Finally, the influent and effluent will be measured using the developed method to determine which wastewater treatment method removes the most triclosan. |
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