5th Annual WSU Celebration of Research and Creative Scholarship
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Poster #65 - The Effect of Metformin on Glycolysis of Lactobacillus Acidophilus Mitchell J. Larsen Faculty Co-author: Myoung E. Lee Metformin is one of the most common drugs used in the treatment of Type II diabetes. Metformin is known to have antihyperglycemic effects, mainly by reduced hepatic gluconeogensis, however its exact mechanism is still not understood. Previous experiments at WSU involving Metformin’s effects have been performed on yeast cells which are simple eukaryotic organisms. The purpose of this research is to understand the effects of Metformin on a simple prokaryotic species, Lactobacillus acidophilus (LA-5). The LA-5 was re-suspended in a phosphate buffer where one sample was given Metformin and the other was not. N2 gas was bubbled in the buffer system to create an anaerobic environment suitable for glycolysis. The two samples were incubated at room temperature for 1 hour. 1-13C-Glucose was added to both samples and then monitored via Nuclear Magnetic Resonance (NMR). The peak height of the disappearance of the reactants, the α and β anomers of glucose, was plotted vs. time. Additionally, the peak height of the appearance of the product, lactic acid, was plotted vs. time. The effects of Metformin on the rate of glycolysis were then studied. |
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