6th Annual Judith Ramaley Celebration of Research and Creative Scholarship
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Poster #91 Affinity Purification of Recombinant Viral Fibroblast Growth Factor and Analysis of Activity
Suad Roble Faculty Mentor: Casey M Finnerty Fibroblast growth factors (FGFs) are typically small (~17-34 kDa) cytokines found in animals ranging from nematodes to humans, and they comprise a large family of growth and differentiation factors with essential functions in development, metabolism, and repair of various tissues and organs. Viral FGFs are mostly found in the family Baculoviridae, which are a diverse family of pathogens that are infectious for arthropods, particularly Lepidoptera. vFGFs are important baculovirus virulence factors. vFGFs appear to induce chemotaxis of cells, allowing the virus to infect these cells and have been spread through the open circulatory system of the insect. We would like to test a vFGF from the baculovirus AcMNPV for mitogenic activity. A maltose binding protein-vFGF fusion protein (MBP-vFGF) was purified via affinity chromatography with an amylose resin column. This fusion protein will be tested for mitogenic activity in Sf9 cell cultures. |
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