5th Annual WSU Celebration of Research and Creative Scholarship
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Poster #74 - GC-MS Identification of unknown constituents in lipid extracts from biota sampled from the Upper Mississippi River Tanner McDaniel Lynn A. Bartsch (UMESC), Michelle R. Bartsch (UMESC), William B. Richardson (UMESC) Faculty Co-author: Thomas Nalli The main goal of this project was to identify major unknown peaks found in lipid extracts from various Upper Mississippi River (UMR) specimens. Collection of specimens and extraction procedures were carried out by scientists at the Upper Mississippi River Environmental Sciences Center (UMESC) in La Crosse, Wisconsin. Standard solutions of fatty acid methyl esters (FAMEs) were analyzed by GC-MS using an Agilent 60-m DB-23 column. Thermodynamic retention indices (TRI) were determined by running the standards at two different oven temperature ramps and using Pro ezGC method Development software (Restek, Bellefonte, PA), to determine optimum GC parameters for UMR specimen analysis. The lipids were extracted with CHCl3/MeOH and methylated with H2SO4/MeOH prior to analysis. Initial analysis made use of GC-FID with most constituents identifiable by retention time. This project sought to determine unidentified peaks thought to be fatty acids, or dimethyl acetals, phytols and phytol derivatives. |
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